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Monday, May 17, 2010
3:00 PM - 4:00 PM
CNLS Conference Room (TA-3, Bldg 1690)

Colloquium

Nucleic Acid Structure and Dynamics: A Spin Label's Perspective

Peter Qin
University of Southern California

In site-directed spin labeling (SDSL), a stable nitroxide radical is attached at a specific location within a macro-molecule, and structural and dynamic information at the labeling site is obtained via electron paramagnetic resonance (EPR) spectroscopy. Our work centers on a family of nitroxide probes, called the R5-series, which can be attached at a specific nucleotide of any given RNA and DNA in an efficient and cost-effective manner. I will present work on using the R5-probes for measuring nanometer distances as well as probing site-specific structural and dynamic features of DNA and RNA.

Host: Chang-Shung Tung, T-6