Whole-genome in vivo monitoring of protein-DNA interactions in Escherichia coli

From Q-bio

The ability to monitor the occupancy of transcription-factor binding sites in vivo is an important prerequisite to modeling and understanding regulatory network dynamics. We have developed a technology for whole-genome identification and monitoring of protein-DNA "footprints" in bacterial genomes. We employed the power of genome-wide analyses to detect underlying complex patterns connecting the set of DNA-protein interactions and the transcription regulatory network. In addition, we are using this technology to gain a better understanding of the structural organization of the bacterial genome in terms of nucleoid proteins and the domains they help to organize.