Quantitatively monitoring the G1 stability in single cell

From Q-bio

G1 (START) checkpoint interprets a flood of signals that influence cell division and cell fate. However, our current knowledge about the G1 checkpoint is mostly qualitative. We built a single cell assay to quantitatively monitor the G1 stability and the kinetic of G1/S transition in yeast cell cycle. By using fluorescent microscopy, the kinetic process of G1/S transition can be visualized in single cell in real time. Coupled with computational modeling, this quantitative assay will be used to elucidate various and compounded factors leading to the G1 checkpoint arrest or bypass.